Sentinel flaps correlate with pro-inflammatory and immunoregulatory mediators of human face transplantation rejection
نویسندگان
چکیده
Abstract Early diagnosis and treatment of immune rejection is critical for face transplants (vascularized composite allografts, VCA). VCA diagnosed by histologic evaluation skin biopsies, yet repeated biopsies are a considerable aesthetic problem. Sentinel flaps, fasciocutaneous radial forearm have been used remote-site monitoring, but the concordance between tissues during remains unclear. With access to world’s largest cohort patients at single center worldwide, we studied 14 paired from sentinel flaps (SF) facial allografts (FA) non-rejection. We previously identified 15 pro-inflammatory genes (including GZMB, HLA-DPB1, IFNG) immunoregulatory SOCS1, CTLA4, IDO1) upregulated in rejection. SF FA had an average correlation coefficient 0.88 0.76 pro- anti-inflammatory gene sets, respectively. During rejection, there was no significant difference expression tissue type (SF versus FA), only one (PDCD1) significantly different. Across all samples, were 64 differentially expressed (DEG) non-rejection, 13 DEG types, 2 when statistically accounting differences driven status (log2FC>1, p<0.05). High throughput sequencing (HTS) CDR3 TCR-β 4 matched pairs mean 9.5 expanded shared T cell clones, indicating that antigens drove both tissues. Together, these data indicate could serve as effective monitoring transplant NIH T32 Training Grant 2T32AR007098-47 Dept. Dermatology, Harvard Medical School RT170025 (P.I. Rachael A. Clark) Department Defense - congressionally Directed Research Programs; "T cells Rejection Vascularized Composite Allografts).
منابع مشابه
Pro-Inflammatory Mediators and Apoptosis Correlate to rt-PA Response in a Novel Mouse Model of Thromboembolic Stroke
BACKGROUND A recent study suggests that patients with persistent occlusion of the middle cerebral artery (MCA) following treatment with recombinant tissue plasminogen activator (rt-PA) have better outcomes than patients with MCA occlusion not receiving rt-PA. We performed a study to elucidate possible mechanisms of this finding in a new model of thromboembolic stroke closely mimicking human pat...
متن کاملIntervertebral disc herniation, spinal nociceptive signaling and pro- inflammatory mediators
متن کامل
Identification and signature profiles for pro-resolving and inflammatory lipid mediators in human tissue.
Resolution of acute inflammation is an active process locally controlled by a novel genus of specialized pro-resolving mediators (SPM) that orchestrate key resolution responses. Hence, it is of general interest to identify individual bioactive mediators and profile their biosynthetic pathways with related isomers as well as their relation(s) to classic eicosanoids in mammalian tissues. Lipid me...
متن کاملPro-inflammatory cytokine predicts reduced rejection of unfair financial offers.
OBJECTIVES This study aimed to examine one of biological correlates, pro-inflammatory cytokine, in rejection of unfair financial offers in the Ultimatum Game (UG), where the division of a sum of money is proposed and the player can accept or reject this offer. METHODS Nineteen participants played 20 trials of the UG as responders, and they were proposed unfair offers in a half of the trials. ...
متن کاملDrusen and Pro-inflammatory Mediators in the Post-Mortem Human Eye
Despite significant advances in treatment, age-related macular degeneration (AMD) remains the leading cause of blindness in the elderly of developed nations [1]. Retinal cells experience functional decline with age as a result of metabolic changes and cumulative oxidative stress [2]. Early AMD is characterized by retinal pigment epithelium (RPE) dysfunction [3]. In the early stages of disease, ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Immunology
سال: 2023
ISSN: ['1550-6606', '0022-1767']
DOI: https://doi.org/10.4049/jimmunol.210.supp.173.25